Figure 12.

Subcellular Localization of GFPAtRAC8 and GFPAtRAC7mV¹⁵ in Dividing and Elongating Cells.

Both GFPAtRAC8 and GFPAtrac7mV¹⁵ were distributed evenly around the cell periphery in dividing and elongating root cells ([A], [B], and [D]) and in developing stomata guard cells ([C] and [E]).

(A) Region of the root tip of a transgenic Arabidopsis plant expressing GFPAtRAC8.

(B) The black rectangle denotes the cell that was observed in detail with the confocal laser scanning microscope. This image is from a projection stack of multiple confocal sections.

(C) Unequal cell divisions (arrowheads) leading to the development of stomata guard cells can be viewed in the cotyledon epidermis of the same transgenic plant shown in (A) and (B).

(D) Elongating root cells from a GFPAtracmV¹⁵-expressing plant.

(E) Cotyledon epidermis of the same plant shown in (D). Arrowheads point to developing stomata guard cells.

Images in (A), (C), (D), and (E) were taken with a wide-field fluorescence microscope. Bars = 20 μm.