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. 2002 Oct;14(10):2431–2450. doi: 10.1105/tpc.005561

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Copyright © 2002, American Society of Plant Biologists

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Figure 5. — Plasmolysis of N. benthamiana Cells Expressing GFPAtRAC7, GFPAtRAC8, and Their Corresponding Double-Cys Mutants.

After plasmolysis, GFP fluorescence was detected in the plasma membrane of cells that expressed GFPAtRAC7 (AtRAC7) or GFPAtRAC8 (AtRAC8). GFP fluorescence had a typical cytoplasmic distribution after plasmolysis of cells that expressed GFPAtrac7mSS^203+206 (Atrac7mSS) and GFPAtrac8mSS^199+205 (Atrac8mSS). All images are from confocal scans performed with a confocal laser scanning microscope. DIC, Nomarsky differential interference contrast image; F, GFP fluorescence image; F+DIC, overlay of fluorescence and DIC images. Arrowheads point to the plasma membrane (PM), cytoplasmic thickening (C), and cell walls (CW). Bars = 20 μm.