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. 2002 Oct;14(10):2431–2450. doi: 10.1105/tpc.005561

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Copyright © 2002, American Society of Plant Biologists

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Figure 6. — Immunoblot Analysis of Subcellular Fractions Containing AtRAC7, Atrac7mSS^203+206, AtRAC8, and Atrac8mSS^199+205.

Detection of GFPAtRAC7 (AtRAC7), GFPAtrac7mSS^203+206 (Atrac7mSS), GFPAtRAC8 (AtRAC8), and GFPAtrac8mSS^199+205 (Atrac8mSS) with anti-GFP monoclonal antibodies after transient expression in leaves of N. benthamiana, centrifugal separation to soluble (S) and insoluble pellet (P) fractions, and separation by SDS-PAGE. The number at left denotes molecular mass in kD. Note the absence of the double-mutant proteins from the insoluble pellet fraction.