Figure 5.

RT-PCR Showing TT16/ABS mRNA Accumulation in Organs of Arabidopsis Plants.

TT16/ABS transcripts were detected after 35 cycles of PCR amplification on reverse transcription products using primers TT16-6 and TT16-Stop (see Figure 3A). PCR products were visualized on a 2.5% (w/v) agarose gel stained with ethidium bromide. The two bands correspond to the two cDNA forms. Transcript levels of the Arabidopsis elongation factor EF1αA4 were used as a control (migrated on a 1% [w/v] agarose gel). Abbreviations for the organs examined are as follows: Bd, floral buds; Fl, full-blown flowers; Sil, immature siliques at different ages of embryo development; Se, seeds; SilV, silique valves; Sd, 4-day-old seedlings; St, stems; Le, rosette leaves; Rt, roots. For silique samples, Sil1 comprises siliques from 1 and 2 days after pollination (dap), Sil2 comprises siliques from 3 to 5 dap, and Sil3 comprises siliques from 6 to 10 dap. Seeds in lane 6 were harvested from the globe to late-torpedo stages of embryo development and removed from the silique valves.