Figure 2.

Subcellular localization of fluorescein-labeled nido- and closo-OPDs in vitro. (a and b) The same set of cells 2 h after coinjection of nido-(CB)₅ (5 μM solution) and rhodamine-labeled BSA (1 mg/ml) into the cytoplasm. When viewed at their characteristic UV wavelengths, nido-(CB)₅ appears in the nucleus (a) and BSA appears in the cytoplasm (b). (c and d) Rapid nuclear accumulation of nido-(CB)₅ (100 μM solution) (c) and nido-(G1)₅ (100 μM solution) (d) within 10 min after cytoplasmic microinjection. (e and f) Long-term nuclear retention of nido-(CB)₅ (100 μM solution) (e) and nido-(G1)₅ (100 μM solution) (f) in the cells 24 h after cytoplasmic microinjection. (g and h) Distribution of OPD in subcellular compartments in digitonin permeabilized cells incubated with nido-(CB)₅ (g) and nido-(G1)₅ (h). (i and j) Subcellular distribution of closo-(G1)₅ in the cells 2 h after microinjection (100 μM solution) (i) and in digitonin permeabilized cells (j).