Table 2.

Confirmation of Microarray Data by Real-Time PCR

Time Point	cDNA Templates	Open Reading Frame Identifier	Function	Microarray	Real-Time PCR
0.5 h		At1g59870	ABC transporter	2.09	2.14
		At1g21100	Similarity to O-methyltransferase1	1.39	1.50
		At5g10210	Putative protein	2.41	7.07
2 h	1	At2g41730	Hypothetical protein	2.01	5.20
	2	At2g41730	Hypothetical protein	6.56	3.10
		At5g59820	Zinc finger protein Zat12	3.56	11.30
		At4g15760	Hypothetical protein	2.72	3.47
4 h		At4g01900	Nonsymbiotic hemoglobin (GLB1)	3.34	3.90
	1	At5g48540	33-kD secretory protein-like	2.82	3.76
	2	At5g48540	33-kD secretory protein-like	2.09	2.28
	1	At3g46600	Hypothetical protein	1.96	2.05
	2	At3g46600	Hypothetical protein	1.48	3.16
		At1g77120	Alcohol dehydrogenase	13.08	4.60
	1	At1g76600	Unknown protein	2.86	2.98
	2	At1g76600	Unknown protein	6.25	6.67
	3	At1g76600	Unknown protein	2.66	2.57
20 h	1	At3g11930	Unknown protein	2.08	2.66
	2	At3g11930	Unknown protein	4.34	21.17
		At2g41730	Hypothetical protein	7.25	5.01
	1	At1g11580	Pectin methylesterase	3.69	12.31
	2	At1g11580	Pectin methylesterase	2.59	2.46
		At4g17190	Farnesyl diphosphate synthase2	3.51	3.49
		At4g20830	Reticuline oxidase	5.27	3.64

Differentially expressed genes were chosen across the four time points and across functional categories. Some genes were tested with two or three cDNA templates, as indicated. Nontransformed ratios are shown for both the microarray and real-time PCR approaches.