Table 2.
Overview of TaqMan/SYBR-Green Primers and Probes Used
| Primer/probe | Gene | Sequence (5′ to 3′) |
|---|---|---|
| C1F | C1 | GCCCTGGCGTCGTTTCT |
| C1R | C1 | TGGACATCTATACGTGTACTTGTTGTCTAC |
| C1Ta | C1 | CTCCGCTGTCAGACGGCCGG |
| LCF | LC | CGGGAGCAGCACAGGAAAT |
| LCR | LC | GTCGCTTTCGCTCCGACAT |
| LCTa | LC | TGGCACTGGCACCAAGAACCACG |
| CYPF | CYP | GAGTGGCTCAACGGAAAGCA |
| CYPR | CYP | CCAACAGCCTCTGCCTTCTTA |
| CYPTa | CYP | ACATCCATGCCTTCAACAACTTGTCCAA |
| PAL_TOM_F | PAL | ATTGGGAAATGGCTGCTGATT |
| PAL_TOM_R | PAL | TCAACATTTGCAATGGATGCA |
| CHS_TOM_F | CHS | TGGTCACCGTGGAGGAGTATC |
| CHS_TOM_R | CHS | GATCGTAGCTGGACCCTCTGC |
| CHI_TOM_F | CHI | GTTTTTCACAAACCAACAGTTCTGAT |
| CHI_TOM_R | CHI | GAAGCAGTGCTCGATTCCATAAT |
| F3H_TOM_F | F3H | CACACCGATCCAGGAACCAT |
| F3H_TOM_R | F3H | GCCCACCAACTTGGTCTTGTA |
| F3′H_TOM_F | F3′H | GCACCACGAATGCACTTGC |
| F3′H_TOM_R | F3′H | CGTTAGTACCGTCGGCGAAT |
| F3′5'H_TOM_F | F3′5′H | GGCAATTGGACGAGATCCTG |
| F3′5'H_TOM_R | F3′5′H | AAGGAACCTCTCGGGAGTGAA |
| FLS_TOM_F | FLS | GAGCATGAAGTTGGGCCAAT |
| FLS_TOM_R | FLS | TGGTGGGTTGGCCTCATTAA |
| DFR_TOM_F | DFR | TCCGAAGACGACAACGGTTT |
| DFR_TOM_R | DFR | TGACAAGCCAAGAGCCGATAA |
| ANS_TOM_F | ANS | GAACTAGCACTTGGCGTCGAA |
| ANS_TOM_R | ANS | TTGCAAGCCAGGCACCATA |
| GT_TOM_F | GT | CGAACGACGAAACACTGTTGA |
| GT_TOM_R | GT | TGCAGCATAGATGGCATTGG |
| RT_TOM_F | RT | CTGGCAATGCAAACAGAGTGA |
| RT_TOM_R | RT | TCGACTTGCGGAAGAGTGAGA |
| ASR1_TOM_F | ASR1 | CCTGTTCCACCACAAGGACAA |
| ASR1_TOM_R | ASR1 | GTGCCAAGTTTACCGATTTGC |
a
Fluorescently labeled TaqMan probes.