Figure 1.

Genomic Structure of FLC and FLC::GUS Reporter Constructs.

Coding and intron sequences are shown as black and white boxes, respectively. The 5′ and 3′ noncoding regions (including 5′ promoter regions) are shown as striped boxes. The start (ATG) and stop (TAA) codons are indicated. Nucleotide numbering is given below each construct, taking the A of the ATG as +1. The segment numbers referred to in the text are indicated at bottom. E, EcoRV; H, HindIII; Sp, SpeI; St, StyI.

(A) Diagram of the FLC gene.

(B) to (F) Diagrams of the FLC::GUS constructs. All constructs were fused to the GUS coding sequence and the 3′ octopine synthase terminator (Dolferus et al., 1994). The end points of the deletions in (D) to (F) are indicated below the diagrams of the constructs.

(B) 6-kb construct.

(C) 2-kb construct.

(D) Δ2.8 construct.

(E) Δ1.3 construct.

(F) Δ1.2 construct.

(G) Region contained within the 6-kb construct.