Figure 5.

Binding of ZmABI4 Protein to Upstream Sequences in ABA-Related Genes.

(A) Sequence alignment of upstream regions of ZmABI4 and ABA-regulated genes containing CE1-like sequences as well as mutated ZmABI4 binding sites used in electrophoretic mobility shift assay analysis. Numbering of sequence location is from the transcription start site for each gene except ZmABI4, which is numbered from the translation start. Sequences of maize ABI4, maize rab28 (Busk et al., 1999), barley HVA1 (Shen et al., 1996), barley HVA22 (Shen and Ho, 1995), maize rab17 (Busk et al., 1997), rice rab16B (Ono et al., 1996), and Craterostigma plantagineum CdeT27 (Michel et al., 1993) are presented.

(B) Alignment of the putative TATA box and the ABI4 binding site of Arabidopsis (AtABI4), maize (ZmABI4), and rice (OsABI4) ABI4 genes. The TATA box and the ABI4 binding site are boxed.

(C) Electrophoretic mobility shift assay analysis of ZmABI4 binding to the CE1-like sequences presented in (A).