Figure 2.

Structural Analyses of the Wild Type and qua1-1 Mutants.

(A) and (B) Cross-sections of the hypocotyls of wild-type (WT) and qua1-1 mutant plants stained with methylene blue after 7 days of growth in the dark in vitro in the absence of sugar.

(C) Loss of cell adhesion in roots of the qua1-1 mutant. Roots of 2-week-old plants grown in vitro with a 16-h-light/8-h-dark regime were stained with FM4-64 and visualized with a confocal microscope. The arrow indicates an epidermal cell that is partially detached from the roots.

(D) to (G) Scanning electron micrographs of wild-type and qua1-1 epidermal cells from hypocotyls ([D] and [E]) or leaves ([F] and [G]). Plants were grown for 2 weeks in vitro with a 16-h-light/8-h-dark regime before analysis. Bars = 50 μm ([D] and [E]) or 100 μm ([F] and [G]).