Figure 5.

Immersion Immunofluorescence of Wild-Type, qua1-1, and qua1-2 Roots Incubated with the Monoclonal Antibodies JIM5 and JIM7.

Roots of 6-day-old seedlings grown in vitro in a 16-h-light/8-h-dark regime were incubated with either the JIM5 or the JIM7 monoclonal antibody. After washes and incubation with a secondary fluorescein isothiocyanate–coupled antibody, roots were visualized with a microscope coupled to a charge-coupled device camera. Negative controls refer to roots incubated with the secondary antibody without previous exposure to the JIM5 or the JIM7 antibody. All images were recorded with the same settings (light intensity, filters, and camera settings) for wild-type, mutant, and background control samples. Ws, Wassilewskija. Bars = 200 μm.