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Lc753–893 binding to synaptic proteins. GST fusion proteins (100 pmol) were immobilized on GSH-agarose beads and incubated for 60 min with His6 Lc753–893 (250 pmol). After extensive washing, the remaining bound proteins were eluted with 15 mM GSH, separated on a 10% SDS acrlyamide gel, transferred to nitrocellulose, and subjected to Western analysis by using anti L-peptide antibodies and ECL detection. His6 Lc753–893 (30 ng) was used as a marker.
