Figure 5.
The activation kinetics of the Lc-type channel is modified by syntaxin and p65. Rates of activation of the Lc channels (τact = 3.9 ± 0.8 ms; ○; n = 16) were recorded in oocytes expressing syntaxin (Sx) (A) (7 ng/oocyte; τact = 6.2 ± 1.5; •; n = 8), p65 (B) (5 ng/oocyte; τact = 4.0 ± 0.5 ms; ▵; n = 12), and p65 (7 ng) and syntaxin (C) (5 ng/oocyte; τact = 4.1 ± 0.8 ms; ⋄; n = 10). Ba2+ currents were evoked from a holding potential of −80 mV in response to a 100-ms pulse to a test potential of +20 mV. (Insets) Leak-subtracted representative traces (on-line subtraction). The dotted lines represent mono-exponential fitting of the normalized traces. Statistical significance was determined by using one-way ANOVA P < 1.7E-8.