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. 2002;14(Suppl):s375–s388. doi: 10.1105/tpc.001065

Figure 2.

Figure 2.

Schematics of CA and DN rop Mutants Used for Functional Analysis.

The DN mutant of GTPase is locked in GDP-bound or nucleotide-free form, and the CA mutant is locked in GTP-bound form. Overexpression of DN mutants blocks Rop activation by sequestering activators, whereas CA mutants are insensitive to the action of RopGAPs and thus permanently activate effectors. Examples shown are those used for the investigation of the function of Arabidopsis ROPs (Kost et al., 1999; Li et al., 1999; Lemichez et al., 2001; Li et al., 2001). X indicates the inhibition of the cycling between GDP and GTP forms by the dominant mutations.