Figure 3.

Rop Regulates Cell Polarity Development and Polar Growth through Recruitment to and Activation at the Site of Action.

Typical cell-shape phenotypes induced by CA-rop, DN-rop, or Rop OX in different cells (Li et al., 1999; Fu et al., 2002; Jones et al., 2002) are shown at left, and Rop localization or its activation is shown at right.

(A) Rop localization to the apical PM region in pea pollen tubes as shown by indirect immunoflurorescence (Lin et al., 1996).

(B) The localization of GFP-tagged RIC1 in tobacco pollen tubes, indicating the localization of GTP-bound active Rop to the apical PM region (Wu et al., 2001).

(C) to (E) The localization of GFP-tagged ROP2 to the future hair forming site in the epidermal cell (C), the tip of swelling prior to the initiation of tip growth (D), and the tip of growing root hair (E) (Jones et al., 2002).

(F) Preferential localization of GFP-ROP2 to the site of lobe formation in expanding leaf epidermal cells (Fu et al., 2002).

All images shown were obtained using confocal microscopy. Scale bars = 10 μm.