Real-time PCR validation of microarray results. (a) Concordance of gene-expression patterns determined by oligonucleotide and PCR amplicon microarrays and real-time PCR. Data shown are the mean ± SD of four to five independent determinations. H4, histone H4; K3H, kynurenine 3-hydroxylase; 14-3-3γ, 14-3-3 protein gamma Ser/Thr PK, serine/threonine protein kinase; CO, cytochrome c oxidase. (b) Real-time PCR analysis of discordant results from oligonucleotide and PCR amplicon arrays. Data shown are the mean ± SD of four to five independent determinations. IP3R, inositiol-1,4,5-trisphosphate receptor; HATP, H+-ATPase; HBCA, heart branched-chain aminotransferase; EH, cytosolic epoxide hydrolase.