Table 2.
Genes induced in symbiosis
| Gene ID | Gene name | Description | Cultured bacteria | Symbiotic bacteria | ||||||||
| M. truncatula | M. sativa | |||||||||||
| V + O2 | TY | VμO2 weak limitation | VμO2 strong limitation | Luteolin | J6-1021 18 dai | J6-1021 8 dai | TE7-1021 | J6-bacA | Sitel-1021 | |||
| Controls | ||||||||||||
| SMa0815 | nifA | Transcriptional activator of nitrogenase genes | 1 | 1.5 | 2.8 (+) | 1.4 | 2.1 | 20 (+) | 24 (+) | 6.0 (+) | 1.9 | 27 (+) |
| SMa0825 | nifH | Nitrogenase Fe protein | 1 | 0.9 | 0.6 | 0.7 | 1.7 | 13 (+) | 13 (+) | 2.7 (+) | 0.6 | 58 (+) |
| SMa0873 | nifN | Nitrogenase Fe-Mo cofactor biosynthesis protein | 1 | 0.9 | ND | 0.7 | 0.9 | 2.4 (+) | 4.2 (+) | 3.5 (+) | 4.8 (+) | 4.4 (+) |
| SMa1225 | fixK1 | Transcriptional activator of fix genes | 1 | 0.8 | 2.1 (+) | 2.4 (+) | 2.3 | 6.1 (+) | 9.6 (+) | 6.6 (+) | 8.3 (+) | 4.2 (+) |
| SMa1220 | fixN1 | Heme b/copper cytochrome c oxidase subunit | 1 | 2.0 | 15 (+) | 17 (+) | 3.7 | 13 (+) | 31 (+) | 5.3 (+) | 19 (+) | 8.8 (+) |
| SMa1213 | fixP1 | Di-heme cytochrome c | 1 | 1.9 | 8.7 (+) | 11 (+) | 3.7 | 11 (+) | 8.9 (+) | 6.7 (+) | 4.6 (+) | 4.4 (+) |
| SMa1226 | fixT1 | Inhibitor of FixL autophosphorylation | 1 | 1.9 | 3.4 | 2.3 | 2.9 | 3.7 (+) | 7.9 (+) | 5.3 (+) | ND | 1.2 |
| SMa1229 | fixL | Oxygen-regulated histidine kinase | 1 | 1.5 | 10 (+) | ND | 1.6 | 18 (+) | 17.5 (+) | 17.5 (+) | 11.5 (+) | 0.5 |
| SMc00819 | katA | Catalase | 1 | 0.6 | 1.1 | 1.0 | 1.3 | 3.9 (+) | 3.8 (+) | 1.9 | 2.0 | 1.9 (+) |
| SMa1077 | nex18 | Hypothetical protein | 1 | 1.3 | 2.4 (+) | 2.6 (+) | 1.2 | 2.3 (+) | 1.5 | 3.5 (+) | 2.3 (+) | 1.4 |
| SMa1079 | tspO | Tryptophan rich sensory protein homolog | 1 | 1.1 | 2.4 (+) | 1.6 | 1.4 | 1.9 (+) | 1.8 (+) | 2.7 (+) | 1.7 (+) | 2.0 (+) |
| SMc01030 | pdhAa | Pyruvate dehydrogenase | 1 | 1.3 | 1.3 | 1.9 | 1.5 | 2.0 (+) | 1.8 | 2.5 | ND | 2.1 (+) |
| New genes induced in bacteroids | ||||||||||||
| SMc02285 | cyaE | Adenylate cyclase | 1 | 1.0 | 1.0 | 0.7 | 0.9 | 1.4 (+) | 2.5 (+) | 1.1 | 2.5 (+) | 1.5 (+) |
| SMc03099 | cyaF1 | Adenylate cyclase | 1 | 1.0 | 0.8 | 0.8 | 0.8 | 1.9 (+) | 2.1 (+) | 1.3 | 1.4 (+) | 1.1 |
| SMb20539 | cyaF6 | Adenylate cyclase | 1 | 1.0 | 1.3 | 0.9 | 0.8 | 3.3 (+) | 3.0 (+) | 2.1 (+) | 2.1 (+) | 2.4 (+) |
| SMa0841 | sma0841 | Transposase (hypothetical protein) | 1 | 1.1 | 0.7 | 0.5 | 0.9 | 3.7 (+) | 8.8 (+) | 3.5 (+) | 3.8 (+) | 2.7 (+) |
| SMb20518 | smb20518 | Unknown function (possible lytic enzyme) | 1 | 0.9 | 0.8 | 0.5 (-) | 1.0 | 2.9 (+) | 3.5 (+) | 1.6 (+) | 2.4 (+) | 1.6 (+) |
| SMc02394 | smc02394 | Virulence associated protein homolog | 1 | 1.2 | 1.2 | 1.1 | 1.0 | 1.8 (+) | 1.0 | 1.2 | 1.8 (+) | 1.7 (+) |
| Genes induced in symbiosis and by luteolin | ||||||||||||
| SMc03785 | ialA | Probable invasion protein | 1 | 1.0 | 4.3 (+) | 2.8 (+) | 9.2 (+) | 2.9 (+) | 2.8 (+) | 1.6 | ND | 3.8 (+) |
| SMc02509 | sitA | Iron transporter | 1 | 1.5 | 1.1 | 0.7 | 12.2 (+) | 3.6 (+) | 4.3 (+) | 3.8 (+) | 4.8 (+) | 4.3 (+) |
| SMc01516 | smc01516 | Unknown function | 1 | 1.0 | 1.5 | 1.2 | 5.7 (+) | 3.5 (+) | 4.8 (+) | 3.5 (+) | 1.5 | 3.0 (+) |
| Other genes induced in M. truncatula but not in M. sativa | ||||||||||||
| SMb20838 | smb20838* | Hemolysin-type calcium-binding protein | 1 | 1.4 | 1.1 | 2.6 (+) | 0.9 | 2.1 (+) | 2.2 (+) | 2.4 (+) | 3.0 (+) | 0.8 |
| SMa0627 | aqpZ2 | Aquaporin | 1 | 1.0 | 1.2 | 0.8 | 1.1 | 2.2 (+) | 1.8 (+) | 1.1 | 1.4 | 0.7 |
| SMc03784 | smc03784 | Unknown function | 1 | 1.3 | 1.2 | 0.8 | 1.0 | 2.7 (+) | 2.1 (+) | 0.8 | 1.2 | 0.9 |
Legend as for Table 1. *In the DNA region amplified, SMb8038 and SMc00287 share > 90% identity. The signal is therefore a combination of the expression of the two genes.