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. 2005 Feb 24;2005(1):31–38. doi: 10.1155/MI.2005.31

Figure 4.

Figure 4

Figure 4

Figure 4

Figure 4

Effects of synthetic polypeptides on PI3-K and p38 MAP kinase expression. Neutrophils were incubated (37°C) with medium control, fMLP (5 × 10−8 M), or 10 nM (Pro-Pro-Gly)5, (Pro-Pro-Gly)10, (Pro-Hyp-Gly)5, or (Pro-Hyp-Gly)10 for 2 hours (PI3-K) or 5 minutes (p38, phospho-p38). (a) PI3-K, (b) p38, (c) phospho-p38, (d) protein expressions were measured using western blotting and quantified by densitometry. Time course of phospho-p38 protein expression in response to (Pro-Pro-Gly)5. Western blots were scanned by densitometry and data presented as relative intensity units. One representative experiment of 3 is shown for each analysis.