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Separation of cytokinin standards and products of enzymatic reactions by HPLC as reported (17). (A) Cytokinin standards. (B) Products of enzymatic reaction after incubating soluble recombinant protein from 10 ml of cell culture with [14C]zeatin (0.8 nmol; 45,000 cpm), UDPglucose (3 mM), ATP (0.5 mM), and MgCl2 (0.05 M) for 2 h. (C) Reaction product (open bar) obtained from B treated with β-glucosidase for 2 h as reported (22), resulting in zeatin (hatched bar).
