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. Author manuscript; available in PMC: 2006 Jul 19.
Published in final edited form as: Biochemistry. 2004 Oct 19;43(41):13165–13174. doi: 10.1021/bi048596g

Figure 3.

Figure 3

Sensorgrams of PutA binding to positively charged lipid vesicles. Panel A: Sensorgrams of proline (5 mM) reduced PutA (10, 20, 40, 80, 160 nM, bottom to top) injected onto a L1 chip coated with EDOPC lipid vesicles. Panel B: Sensorgrams of oxidized PutA (20, 40, 80, 160, 320 nM, bottom to top) injected onto a L1 chip coated with EDOPC lipid vesicles. For all sensorgrams, the association phase (a) corresponds to the injection of the PutA sample at 60 μl/min for 120 s and the dissociation phase (b) corresponds to the flow of HEPES-N buffer at 60 μl/min for 300 s. The data were fit by global analysis to a 1:1 Langmuir binding isotherm. Signals from the control surface have been subtracted.