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. Author manuscript; available in PMC: 2006 Jul 19.
Published in final edited form as: Biochemistry. 2004 Oct 19;43(41):13165–13174. doi: 10.1021/bi048596g

Figure 5.

Figure 5

Gel mobility shift assay of the oligonucleotide binding site complexed with PutA. IRdye-700 labeled 21-bp duplex DNA (5 nM) and varying concentrations of PutA (0-400 nM dimer) were incubated in binding mixtures (HEPES-N buffer, pH 7.4) containing 100 μg/ml of nonspecific calf thymus DNA at 20 °C for 20 min. The complexes were separated using a nondenaturing polyacrylamide gel (8%).