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. Author manuscript; available in PMC: 2006 Jul 19.
Published in final edited form as: Circ Res. 2006 Mar 2;98(6):793–800. doi: 10.1161/01.RES.0000216071.87981.16

Figure 3.

Figure 3

The IL-1α promoter is regulated by calcium and calcineurin. Porcine aortic endothelial cells were transfected with vectors containing a luciferase reporter regulated by various regions of the porcine IL-1α gene promoter. The cells were stimulated with 1 μM ionomycin for 6 hours to mobilize intracellular calcium and activation of the IL-1α gene promoter was measured by luciferase assay of cell lysates.

(A) Ionomycin stimulates porcine IL-1α promoter activity. The calcium-responsive region of the IL-1α gene is an element approximately 163 base-pairs 5' of the IL-1α gene transcriptional start site.

(B) The nucleotide sequence of the calcium-responsive element within the IL-1α gene promoter.

(C) The calcium-responsive element of the IL-1α gene promoter requires calcineurin for activation. Porcine aortic endothelial cells were transfected with a luciferase reporter vector regulated by the calcium-responsive element of the IL-1α gene promoter. Transfected cells were treated for 2 hours with 1 μM cyclosporine A (CsA) or with 100 nM FK506 and then stimulated with ionomycin. Cyclosporine A and FK506 reduced ionomycin-stimulated luciferase production.

These results indicate that the IL-1α gene is regulated by a 163 base-pair calcium- and calcineurin-responsive element near the IL-1α transcriptional start site.