Figure 7.
Complement and other pore-forming peptides stimulate NF-κB-binding to the IL-1α gene promoter. Porcine aortic endothelial cells were incubated for four hours with PBS (Un) or with human complement (Co) or melittin (Mel), polypeptides that form pores in cell membranes, or with IL-1α. Nuclear NF-κB proteins bound to the 32P-labeled porcine IL-1α gene promoter probe was detected by electrophoretic mobility shift assay.
(A) Melittin and complement stimulate NF-κB-DNA binding activity in the nuclei of endothelial cells.
(B) Complement stimulates DNA binding of NF-κB components p65 and p50.
(C) Complement-stimulated NF-κB activity requires calcineurin. FK506 abolished complement-stimulated appearance of NF-κB in cell nuclei.
These results show that complement and other pore-forming polypeptides stimulate calcineurindependant NF-κB appearance in nuclei of endothelial cells.