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IgG1 and IgG2a levels in mice of Fig. 5 immunized with yeast or plant-cell HBsAg. The serum from each mouse was diluted 1:100 for reaction with yeast rHBsAg. The IgG1 and IgG2a were reported as a mean of OD420 from five mice of each group. The IgG1/IgG2a ratio was calculated by dividing the mean OD of IgG1 by that of IgG2a. ND, not determined when OD420 of either IgG1 or IgG2a was <0.05.
