(a) Dot blot hybridization of human mPR α
(testicular), β (brain), and γ (kidney) mRNA probes with human
multiple tissue arrays (CLONTECH). Only lanes displaying strong
hybridizations with each of the probes are shown. Lanes 1–3 (various
regions of CNS): b1 and g1, cerebral cortex; a2 and b2, cerebellum; e2,
caudate nucleus; c3, thalamus; d3, pituitary gland; e3, spinal cord.
Lane 4: regions of heart. Lanes 5 and 6 (gastrointestinal tract): h5,
a6, and b6, colon. Lane 7 (kidney, hemopoietic, and lymphatic tissues):
a7, kidney. Lane 8 (reproductive tissues): b8, placenta; d8, uterus;
f8, testis; g8, ovary. Lane 9: liver and endocrine glands. Lane 10:
lymphomas and carcinomas. Lane 11 (fetal tissues): c11, fetal kidney.
(b–e) Northern hybridization of human
α, β, and γ mRNA probes with human multiple tissue Northern blots
(CLONTECH).