Table 1.
Oligonucleotides and plasmids used in this study.
| PCR products/Plasmids | Forward and Reverse primers/Description |
| 1163 bp | exon15-53F (5'-CGTTCTCCCCATGAACTTACA) |
| exon15-1215R (5'-AGGGTCTAGGCTGCAAGAAAG) | |
| 1374 bp | intron0F-141 (5'-ACCCCAGCAGTGTGTGAAGAG) |
| intron1R-6537 (5'-CCCACCCCCAGATCAATACTC) | |
| 1926 bp | 1exonF (5'-GGGGGCGCCGGGACTATGTCG) |
| 15exonR-c1934 (5'-TCTCCCGGCCTCCCATCCCAA) | |
| 2158 bp | 46714F (5'-AGAAGTTCTGGTGGGAAGGAA) |
| 48871R (5'-CAGACCCTGGACTAGATGCTG) | |
| 2299 bp | 48538F (5'-TAAGTGGGGGATGAGTCCAG) |
| 50836R (5'-CCCAGATTCCAGCATCCTAA) | |
| 2628 bp | 17595F (5'-GGCGCAGAGGCTAAGAATAAG) |
| 20202R2 (5'-CCCCCAGCCTCCAGGATGTTC) | |
| 2881 bp | exon15-53F (5'-CGTTCTCCCCATGAACTTACA) |
| exon15-2933R (5'-CCACACAGGAGTCAGGAATGT) | |
| 3588 bp | F8256K (5'-GAGAGAGGTACCCCCGTGACTCCTGGTGGCTGC) |
| R11843K (5'-AATTCGGTACCCCTGGTCACCGGGTTCTCCTA) | |
| 4709 bp | UA1F1979 (5'-CGCACACACCGCCAACTGTTC) |
| UA1R6667 (5'-CCTGGTCACCGGGTTCTCCTA) | |
| 4970 bp | UAP-15639 (5'-GAGTGGCCCTTATGTACCGAC) |
| 1R-20591 (5'-GGGCAACCGGCTCTTGAC) | |
| ATGc | QC20060F (5'-CGCAGGAGCCGAGCTCGGAGTACCAGGTAT) |
| QC20060R (5'-ATACCTGGTACTCCGAGCTCGGCTCCTGCG) | |
| ATGm | QC19934F (5'-GCGCCGGGACTAGTTCGCGGGCGCGG) |
| QC19934R (5'-CCGCGCCCGCGAACTAGTCCCGGCGC) | |
| NFY-868 | NFY-868F (5'-GACGCTGCGCTGAAAAGCTGGGGGCGGGGC) |
| NFY-868R (5'-GCCCCGCCCCCAGCTTTTCAGCGCAGCGTC) | |
| Ebox-952 | E47-952F (5'-GGGCGCGGAGCCATTCGCGCGCCGCTCTATTC) |
| E47-952R (5'GAATAGAGCGGCGCGCGAATGGCTCCGCGCCC) | |
| 139 bp (MSP)2 | M19571F 5'-GTAGAGATTGAGGGTTGTTGATTC |
| M19709R 5'-CGAAACCAAATTTATAAATATACGCT | |
| 142 bp (MSP)2 | U19571F 5'-GTAGAGATTGAGGGTTGTTGATTT |
| U19712R 5'-CCTCAAAACCAAATTTATAAATATCACT | |
| pCR1163 | 1163 bp PCR fragment from NALM6 cloned into pCR2.1 TOPO |
| pCR1374 | 1374 bp PCR fragment from BM cloned into pCR2.1-TOPO |
| pCR2158 | 2158 bp PCR fragment from NALM6 cloned into pCR2.1-TOPO |
| pCR2299 | 2299 bp PCR fragment from NALM6 cloned into pCR2.1-TOPO |
| pCR2628 | 2628 bp PCR fragment from BAC RCPI-11 cloned into pCR2.1-TOPO |
| pCR2881 | 2881 bp PCR fragment from NALM6 cloned into pCR2.1-TOPO |
| pCR4.7A1b | 4709 bp PCR fragment from BM cloned in pCR2.1-TOPO |
| pCR4970 | 4970 bp PCR fragment from BAC RCPI-11 cloned into pCR2.1-TOPO |
| pFPGS-cDNA | 1926 bp PCR from NALM6 cloned into pCR2.1-TOPO |
| pGL1163-2628 | 1163 bp Acc65I fragment PCR from pCR1163 cloned in pGL2628ATGm/c |
| pGL1374 | 1374 bp HindIII PCR fragment from pCR1374 cloned into pGL3 |
| pGL2158-2628 | 2158 bp Acc65I PCR fragment from pCR2158 cloned in pGL2628ATGm/c |
| pGL2256 | 2316 bp HindIII fragment from pCR4.7-A1b cloned into pGL3 |
| pGL2299-2628 | 2299 bp Acc65I PCR fragment from pCR2299 cloned in pGL2628ATGm/c |
| pGL2628 | 2628 bp SacI-EcoRV fragment from pCR2628 cloned into pGL3 |
| pGL2628-ATGc | cytosolic ATG mutagenized to TCG (ATGc) in pGL2628 |
| pGL2628-ATG | mitochondrial ATG mutagenized to AGT (ATGm) in pGL2628 |
| pGL2628-ATGm/c | cytosolic ATG mutagenized to TCG (ATGc) in pGL2628-ATGm |
| pGL2881-2628 | 2881 bp Acc65I PCR fragment from pCR2881 cloned in pGL2628ATGm/c |
| pGL3588-2628 | 3588 bp Acc65I PCR fragment from pCR4.7A1b in pGL2628ATGm/c |
| pGL4689 | 4708 bp EcoRV-BamHI fragment from pCR4970 cloned into pGL3 |
| pGL868NFY-ATGm/c | NFY-868 mutation in pGL2628-ATGm/c |
| pGL952NFY-ATGm/c | Ebox-952 mutation in pGL2628-ATGm/c |
1Nucleotides substituted are in bold italic. 2 MSP, Methyl-Specific PCR BM, normal human bone marrow genomic DNA.
To construct pGL1163-2628, pGL2158-2628, pGL2299-2628, pGL2881-2628, and pGL3588-2628, a sequence (5'-AATTCGGTACC) containing the Acc65I restriction site (underlined) was added to the 5'-end of the oligonucleotide primers used for the PCR amplification.