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. 2006 Jul 21;2(7):e71. doi: 10.1371/journal.ppat.0020071

Figure 4. SHIP Negatively Regulates F. novicida–Induced Akt Activation.

Figure 4

BMMs from SHIP+/+ and SHIP−/− littermate mice were infected for the times indicated in the figure.

(A) Protein-matched lysates were probed with antibodies specific for phosphorylated Erk (Anti-pErk), p38 (Anti-pp38), and JNK (Anti-pJNK). All membranes were reprobed with actin antibody (Anti-Actin).

(B) Protein-matched lysates were probed with phospho-specific antibody for Akt (Anti-pSer Akt; upper panel). The membranes were reprobed with total Akt antibody (Anti-Akt; middle panel), and with SHIP antibody (Anti-SHIP; lower panel). These data are representative of three independent experiments.