. 2006 Jun 28;397(Pt 2):369–375. doi: 10.1042/BJ20060026

Table 2. Effects of pH and DEPC on the kinetics of the Na⁺-dependence of the SNAT2 transporter overexpressed in Xenopus oocytes.

[³H]Serine (0.5 mM) uptake was performed with NaCl concentrations of 0–100 mM at the indicated pH as illustrated in Figure 5. DEPC pre-treatment was for 10 min at 2 mM DEPC as described in the text. Values shown are means±S.E.M calculated from iterative curve-fitting (using GraphPad Prism software) to experimental data of the type shown in Figure 5, using data from four oocyte batches. K_0.5^Na⁺ values refer to the [Na⁺] required for half-maximal activation of 0.5 mM serine transport. V_max^Na⁺ values refer to the maximum rate of 0.5 mM serine transport supported at saturating external Na⁺ concentrations.

Conditions	K_0.5^Na⁺±S.E.M. (mM Na⁺)	V_max^Na⁺±S.E.M. [pmol of serine·oocyte⁻¹·(30 min)⁻¹]
pH 8	5.5±0.9	422±16.9
pH 7	19.8±2.9^*	209±11.8^*
pH 6	68.4±26.5^*	123±38.2^*
pH 8/DEPC	3.7±1.1	164±9.5
pH 7/DEPC	6.1±1.8	132±10.2^*

*P<0.05 (Student's unpaired t test) compared with respective control (pH 8 or pH 8/DEPC).

Table 2. Effects of pH and DEPC on the kinetics of the Na+-dependence of the SNAT2 transporter overexpressed in Xenopus oocytes.

Table 2. Effects of pH and DEPC on the kinetics of the Na⁺-dependence of the SNAT2 transporter overexpressed in Xenopus oocytes.