Figure 7. The mutant p53^F270A/6KR protein is ubiquitinated in vivo.

(A) p53^6KR mutant is ubiquitinated in an MDM2-dependent manner. H1299 cells were co-transfected with wild-type or mutant pcDNA-p53 (0.5 μg) and with or without pCMV-MDM2 (1.5 μg). (B) Comparison of wild-type p53, p53^F270A and p53^6KR mutant ubiquitination using anti-p53 antibodies. The same cell lysates from (A) was examined with different anti-p53 antibodies. (C) Map positions of the monoclonal antibodies DO-1, DO-12, PAb421 and ICA-9.