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. 2006 Jun 28;397(Pt 2):329–336. doi: 10.1042/BJ20052002

Figure 1. NOS regulation of ERK1/2 activity.

Figure 1

(A) HEK-293 cells grown in normal growth medium were treated with or without 10 μM A23187 for 15 min to 2 h. Active ERK1/2 was assayed by immunoblot analysis using the anti-ppERK1/2 antibody. (B) N293 cells grown in medium containing various concentrations (1, 50, 100 or 500 μM) of L-arginine were treated with or without 10 μM A23187 for 2 h, and active ERK1/2 was analysed by immunoblotting using the anti-ppERK1/2 antibody. The results shown are representative of three independent experiments. Anti-ERK1 immunoblots are included as a protein loading control.