Skip to main content
. 2006 Jun 28;397(Pt 2):329–336. doi: 10.1042/BJ20052002

Figure 2. NO• inhibition of ERK1/2 activity is primarily through H-Ras.

Figure 2

(A) Control HEK-293 cells were transfected with constitutively active MKK1, Raf-1 (Raf-1 BXB) or H-Ras (Ras V12) mutant constructs and grown under normal growth conditions for 24–48 h. The upper and lower panels show immunoblots for ppERK1/2 and total ERK1 respectively. (B) N293 cells were transfected with constitutively active MKK1, Raf-1 or H-Ras mutant constructs and were grown under normal growth conditions. Cells were stimulated with 10 μM A23187 and harvested 2 h later. The upper and lower panels show immunoblots for ppERK1/2 and total ERK1 respectively. (C) Ratio of ppERK1/2 to total ERK1 as determined by densitometry. Data represent the means±S.D. for three independent experiments. * P<0.05.