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. 1999 Jan 5;96(1):296–301. doi: 10.1073/pnas.96.1.296

Figure 1.

Figure 1

(A) Position of the FIS2 locus on chromosome II. The relative position of the FIS2 locus and restriction fragment length polymorphism markers m323, mi227, YUP9D3, YUP11D2, YUP11A7 left (L) and right (R) ends, and the BAC 26D2 fragment 5BC are shown. (B) The position of the DsG insertion and the two cDNAs are shown. Asterisks (∗) mark the locations of the mutations in the three ethyl methanesulfonate-induced fis2 alleles. E, EcoRI; B, BamHI. (C) Complementation of fis2 with the cosmid 18H1. The silique on the left is from a FIS2/fis2 plant showing a ratio of 50:50 of normal and fis seeds. On the right is a silique from a FIS2/fis2 plant with an introduced 18H1 FIS2 segment. The ratio of normal to fis seeds is now 75:25 as expected for the complementation of a gametophytic mutation (see data in Table 1). ○, fis seeds; •, FIS seeds. (D) Southern blot of Arabidopsis L.er genomic DNA digested with either BamHI, BglII, or ClaI and hybridized with the 2.38 kb FIS2 cDNA insert.