Table 1.
Ratio of normal:arrested seen in FIS/fis2 plants segregating for the cosmid 18H1
| Line | KanR
progeny (+18H1)
|
KanS progeny
(−18H1)
|
||
|---|---|---|---|---|
| Ratio | X2 | Ratio | X2 | |
| 1 | 179:57 | 0.051 | Not tested | – |
| 6 | 153:55 | 0.23 | Not tested | – |
| 7 | 139:47 | 0.01 | 131:119 | 0.576 |
| 10 | 210:73 | 0.1 | 54:61 | 0.43 |
| Control C24 | NA | – | 211:216 | 0.058 |
KanR plants contain the cosmic and KanS do not. Each line is derived from an independent transformation event. Expected ratios for KanR and KanS plants are 75:25 and 50:50, respectively, if the 18H1 cosmid complements the fis2 mutation. Control cross with C24 shows the segregation of fis2 without complementation. NA, not available.