Table 1.
DNA primers used in the cloning and QPCR analysis of harbor seal target genes.
| Method | Gene | Primer name | GenBank accession no. | Amplicon size (bp) | Primer sequences |
|---|---|---|---|---|---|
| Cloning | Ribosomal protein L8a | UL8up | DQ212693 | 126 | 5′-GGTGTGGCTATGAATCCTGT-3′ |
| L8–2dn | 5′-ACGACGAGCAGCAATAAGAC-3′ | ||||
| Ribosomal protein L8 | PV3 | DQ212694 | 602 | 5′-CCGCCATGGGCCGTGTGATC-3′ | |
| PV4 | 5′-CGTACTCGTGGCCAGCAGTT-3′ | ||||
| TR-α | PV6 | DQ212695 | 631 | 5′-TGCTGCATTATCGACAAGATCAC-3′ | |
| PV8 | 5′-GTGACTTGCCCAGTTCAAAGATGG-3′ | ||||
| TR-β | PV12 | DQ212696 | 801 | 5′-TATTCCTGTAAATATGAAGG-3′ | |
| PV16 | 5′-GTAATTGATATAGTGTTCAAA-3′ | ||||
| QPCR | TR-α | PV19 | — | 231 | 5′-CGACGGAAGGAGGAAATG-3′ |
| PV20 | 5′-GATCTTGGTAAACTCGCTGAA-3′ | ||||
| TR-β | PV30 | — | 425 | 5′-AGAGGCTGGCAAAGAGGA-3′ | |
| PV31 | 5′-ACTTTCTGGGTCATAGCG-3′ |
a
These primers were also used for QPCR analysis.