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. 2003 Feb 24;100(5):2290–2295. doi: 10.1073/pnas.0435715100

Figure 4.

Figure 4

Effect of the E134Q mutation on Ala replacement mutants. (A) Spectra of purified recombinant pigments at pH 7.5 obtained by regeneration of E134Q or Ala replacement/E134Q double mutants with 11-cis-retinal or 9-dm-retinal before (solid lines) and after (shaded gray) illumination with orange light. Broken line shows spectrum of 9-dm E134Q/N302A at pH 8.5 after illumination. (B) Light-dependent Gt-activation rates of rhodopsins containing 11-cis-retinal (black) or 9-dm-retinal (gray) were determined from GTP[γS] uptake by Gt and are presented relative to the rate of WT reconstituted with 11-cis-retinal. Values from left to right are 97.3%, 93.9%, 37.7%, 41.0%, 122.7%, 135.3%, 2.0%, 6.2%, 11.0%, and 17.6%.