Figure 5.

Ry and THI functionally uncouple IP₃R1 and RyR1 in IDCs. (A) Ca²⁺ transients elicited by ATP (20 μM, 5 sec) applied to IDCs that were preincubated either with buffer (top trace), 100 μM Ry for 4 hr (second trace), 100 nM THI (5 min; third trace), or 100 μM Ry (4 hr) + 100 nM THI (5 min) (bottom trace). Traces are mean responses ± SE and represent one of two experiments. (B–E) Statistics (mean ± SE) for time to peak (B), time to baseline (C), percent baseline elevation (D), and percent ATP responders (E) derived from the cells shown in (A). Neither Ry nor THI alone or combined alter time to peak response toward 20 μM ATP (B). However, Ry and/or THI significantly shortened the recovery time to baseline (C), persistently elevated the recovery baseline for resting [Ca²⁺] (D), and recruited more ATP-responsive DCs (E). Results were replicated in three independent experiments.

*p < 0.05. **p < 0.01.