HPLC analysis of culture extracts of the
cloQ− and cloI−
mutants. (A) Clorobiocin standard, wild type,
cloQ− mutant, and
cloQ− mutant fed with ring A. Detection was
at 340 nm. The identity of clorobiocin ([M −
H]− = 695) was confirmed by LC-ESI-CID. Mass
spectroscopic fragments are indicated. (B) Clorobiocin
standard, wild type, and cloI− mutant.
Detection was at 340 nm. (C) Ring A standard, wild type,
and cloI− mutant. Detection was at 254 nm.
The identity of ring A ([M − H]− = 205) was
confirmed by LC-ESI-CID. Mass spectroscopic fragments are indicated.