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Purification of CloQ after overexpression as a fusion protein with GST. The SDS/12% polyacrylamide gel was stained with Coomassie brilliant blue. Lane 1, molecular mass standards; lane 2, total protein after isopropyl β-D-thiogalactoside induction; lane 3, soluble protein after induction; lane 4, eluate after thrombin treatment.
