Figure 3.

Clustering analysis in 257 strains and isolates with the two panels of markers (MLVA-15), genotypes 1 to 68. In the columns the following data are given from left to right : the DNA batch, the genotype, the strain ID including the name of the institution of origin (“BCCN” = Brucella Culture Collection from Nouzilly, INRA, Nouzilly, France ; “BfR” = Federal Institute for Risk Assessment, BfR, Berlin, Germany ; “REF” = the 21 reference and marine mammal strains, prepared by BCCN ; “vacc.” = vaccine strain ), year of isolation, host and geographic origin when known, species and biovar (when relevant). The first genotype number (going from 1 to 204) is the MLVA-15 genotype number. The second (for instance 1.1) indicates the panel 1 genotype number (from 1 to 51) followed by the panel 2 genotype number (from 1 to 200). The corresponding genotyping data can be found in the additional file [see Additional file 1]. Wherever possible, the more precise geographic origin within a country is indicated (for instance France (03) is a strain originating from the French department number 03 (Allier) in the centre of France). The first part of the clustering of the 257 isolates in 204 genotypes is presented. It comprises 68 genotypes, corresponding to B. ovis, B. canis, B. suis biovar 1, 3, 4, and part of the B. suis biovar 2 isolates. The colour code used is as shown in Figure 1.