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. 2006 Apr 20;7:215. doi: 10.1186/1471-2105-7-215

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Copyright © 2006 Sioson et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Overall flow of the statistical analyses of microarray data. Data input is in GPR format and provides the MIV for each spot. TM4 analysis requires ExpressConverter to generate MEV format containing the IIV for each spot. The normalization steps performed by MIDAS are T (total intensity normalization), L (lowess normalization), S (standard deviation regularization), and F (low intensity filter). Differential gene expression is obtained from the Expresso GP model, the Expresso GOT model (Experiment 2 only), and the t-test in MEV.