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Tertiary structure of monomerized λ Cro variants. (A) Near-UV CD spectrum of A33W/F58D Cro at 20°C (100 μM protein/50 mM Tris, pH 7.5/250 mM KCl/0.2 mM EDTA). (B) Thermal denaturation of A33W/F58D as monitored by CD at 222 nm (♦, 10 μM protein/50 mM Tris, pH 7.5/250 mM KCl/0.2 mM EDTA) and 280 nm (⋄, 100 μM protein/50 mM Tris, pH 7.5/250 mM KCl/0.2 mM EDTA).
