Table 1.
Structural parameters obtained from Δ1–52PKG-Iβ scattering experiments
| cGMP:PKG | Rg, Å | Dmax, Å | I0 |
|---|---|---|---|
| 0 | 29.4 ± 0.1 | 90 | 29,000 ± 200 |
| 0.56:1 | 31.1 ± 0.5 | 100 | 18,300 ± 300 |
| 1.47:1 | 36.3 ± 0.6 | 120 | 19,100 ± 400 |
| 1.98:1 | 40.1 ± 0.7 | 130 | 19,400 ± 400 |
| 9.4:1 | 41.4 ± 0.5 | 140 | 35,100 ± 400 |
The estimated molecular mass values determined from I0/c for the cGMP:PKG ratios between 0 and 2 gave an average of 68,000 Da ± 15%. The sample measured with excess cGMP (9.4:1) gave an estimate that was inflated by 30%, indicative of an amount of aggregation involving up to 26% of the Δ1–52PKG-Iβ monomers.