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. Author manuscript; available in PMC: 2006 Oct 13.
Published in final edited form as: Nature. 2006 Apr 13;440(7086):954–958. doi: 10.1038/nature04652

Figure 2.

Figure 2

Reversibility of mitotic exit requires preservation of Cyclin B. a, A Xenopus S3 cell treated with 100 ng/ml nocodazole at time 0. At 30 min after nuclear envelope breakdown, nocodazole was removed and the cell was arrested at metaphase with MG132. Flavopiridol at 5 μM was added at 1:04:00 and removed 25 min later after mitotic exit. The complete video sequence is available as Supplementary Video 6. Bar = 10 μm. b, Experimental protocol employed for producing samples from Hela cells at various stages of induction and reversal of mitotic exit. c, Samples were analyzed for mitotic index and blotted for the indicated proteins.