Table 1.
Measurement of scrapie infectivity in fish tissues by mouse bioassay. Brains from recipient mice inoculated with turbot or trout tissues from either oral or multiple parenteral routes were assayed for PrPSc by Western blot. Whenever samples were available, histology and/or immunohistochemistry was performed. Asterisks indicate mice contributing to the lesion profiles curve of the fish-passaged 139A (Figure 3)
| A. Tissues from infected fish via the oral route | ||||||||
| Turbot | Trout | |||||||
| Days after infection | Muscle | Intestine | Brain | Spleen | Muscle | Intestine | Brain | Spleen |
| 1 | 0/10 | 0/8 | 0/8 | - | 0/10 | 1/8 | 0/5 | - |
| 15 | 0/7 | 0/8 | 0/6 | - | 0/10 | 0/8 | 0/13 | - |
| 30 | 0/10 | 0/8 | 0/10 | - | 0/8 | 0/8 | 0/9 | - |
| 60 | 0/9 | 0/6 | 0/8 | - | 0/7 | 0/6 | 0/6 | - |
| 90 | 0/7 | 0/8 | 0/9 | - | 0/10 | 0/11 | 0/10 | - |
| B. Tissues from infected fish via multiple parenteral routes | ||||||||
| 15 | - | - | 2/8 | 4/7* | - | - | 0/9 | 1/9* |
| 90 | - | - | 1/7* | 0/8 | - | - | 0/11 | 0/11 |