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. 2003 Feb 24;100(5):2420–2425. doi: 10.1073/pnas.0438031100

Figure 1.

Figure 1

Induction of p73 by cisplatin in MMR-deficient cells. (A) Deficiency of MLH1, PMS2, and MSH2 affected induction of p73 by cisplatin. (Upper) The indicated cell lines were treated with 25 μM cisplatin for 48 h, and 30 μg of total protein was analyzed by immunoblotting. MMR proteins are displayed to confirm the deficiency. β-Actin is included for the loading control. (Lower) Two hundred micrograms of HEC1A cell lysates collected at the indicated time with or without 25 μM cisplatin was probed for p73. (B) p73 and p53 protein level in HCT116 and HCT116 3 (6) cells that were treated with the indicated doses of cisplatin (12.5, 25, or 50 μM cisplatin) for 48 h.