Table 1.
Characterization of f-MΦ and s-MΦ
| Relative fluorescence intensity
|
||
|---|---|---|
| f-MΦ | s-MΦ | |
| Surface antigens | ||
| MAC-1 | 78 ± 15 | 81 ± 12 |
| HLA-DR | 19 ± 5 | 102 ± 43 |
| HLA-DQ | 21 ± 8 | 91 ± 27 |
| CD1a | 1 | 12 ± 4 |
| CD14 | 129 ± 27 | 155 ± 22 |
| CD34 | 78 ± 17 | 18 ± 5 |
| CD40 | 51 ± 24 | 48 ± 19 |
| CD45 | 146 ± 24 | 162 ± 41 |
| CD83 | 1 | 1 |
| Cytokine production | ||
| IL-1β | 84 ± 27 | 83 ± 15 |
| IL-6 | 45 ± 20 | 65 ± 16 |
| IL-10 | 9 ± 6 | 56 ± 9 |
| IL-12 p70 | 52 ± 27 | 54 ± 8 |
| TNF-α | 29 ± 13 | 65 ± 18 |
| TNF-RI | 25 ± 7 | 34 ± 15 |
| TNF-RII | 9 ± 4 | 63 ± 27 |
| Adipocyte markers | ||
| Lipids | 14 ± 11 | 157 ± 12 |
| Leptin production | 23 ± 7 | 88 ± 16 |
| PPARγ2 | 20 ± 6 | 110 ± 32 |
| Functional indicators | ||
| Phagocytosis | 189 ± 21 | 197 ± 23 |
| Lymphocyte stimulation, Abs540* | 0.76 ± 0.05 | 0.15 ± 0.03 |
| Cytotoxicity, % | 10 ± 4 | 70 ± 8 |
Relative fluorescence intensity was examined by quantitative ratio imaging microscopy. Stimulation of lymphocyte proliferation was performed by using a 10:1 macrophages-to-lymphocytes ratio, and cytotoxicity was performed by using a 5:1 macrophages-to-target cell ratio (10, 12).
*
Abs540, optical absorbance at 540 nm.