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Daisy, a Jurkat-derived T cell line, inducibly overexpresses FAP48. Daisy cells were induced by 0.5 μg/ml doxycycline (Dox) to overexpress FAP48 over 7 days. Whole-cell extracts (50 μg each) of noninduced (lane 1) and induced (lane 2) cells and Jurkat tet-on cells (lane 3) were analyzed by Western blotting with the anti-FAP48 antiserum 766.
