Figure 3.

FAP48 increases IL2 synthesis in activated Daisy T cells. (A) Jurkat tet-on cells were incubated over 4–6 days with doxycycline and Daisy cells for 6 or 8 days. Cells were synchronized overnight and subsequently stimulated with PMA/ionomycin for 24 h. Cell-free supernatants were analyzed for IL2 production by ELISA (n = 3–8). (B) Daisy cells, incubated without or with doxycycline over 8 days, were treated as described for A in the absence or presence of 1 or 5 μM rapamycin during the stimulation. Subsequently, IL2 production was measured (n = 3). Data of IL2 production by drug-treated cells are expressed as mean (±SE) percentage of that from untreated cells (considered as 100%).