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. 2003 Feb 25;100(5):2456–2461. doi: 10.1073/pnas.0138064100

Figure 2.

Figure 2

Loss of repression leads to defects in cell cycle control and deregulation of E2F target gene expression. (A) WT (+/+), E2f4−/−, DKO, and TKO MEFs were grown and analyzed at 0, 4, 6, and 8 days of confluence for expression of E2F responsive genes. Gene expression was quantitated by using the loading control (ARP PO). (Bi) WT (+/+), (Bii) DKO, (Biii) TKO, and (Biv) E2f4−/− MEFs were grown and maintained at confluence for 8 days and then pulsed with BrdUrd for 24 h. The percentage of cycling cells was based on counting 300 DAPI (4′,6-diamidino-2-phenylindole)-positive nuclei for each genotype.