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. 2003 Feb 25;100(5):2456–2461. doi: 10.1073/pnas.0138064100

Figure 3.

Figure 3

Analysis of E2F4-mediated adipocyte repression. (A) E2f4−/−;p107−/− and E2f4−/−;p130−/− MEFs were induced to differentiate in response to hormones for 2 wk, stained with Oil Red O, quantitated, and compared with WT (+/+), E2f4−/−, and TKO MEFs. (B) WT, E2f5−/−, E2f4−/−, and E2f4−/−;E2f5−/− MEFs were induced to differentiate in response to hormones for 2 wk and analyzed as in A. (C) Full-length or a C-terminal truncated (E2F4ΔC) E2F4 were expressed in control and E2f4−/− MEFs by retroviral-mediated infection. (D) Infected cells were induced by hormones to differentiate for 2 wk and analyzed as above. (E) WT (+/+), E2f4−/−, Rb−/−, and E2f4−/−; Rb−/− MEFs were differentiated in response to hormones for 2 wk and analyzed as above.